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Monday, February 12th, 2018
Table of Contents

1 Introduction
5 PMID
 [F] Diseases Research  / PubMed Research Articles  /
An alcohol withdrawal test battery measuring multiple behavioral symptoms in mice.

PubMed

 

Resource

Alcohol (Fayetteville, N.Y.) Sep ; 68()

Authors

Metten P1; Schlumbohm JP2; Huang LC3; Greenberg GD4; Hack WR5; Spence SE6; Crabbe JC7;

Author Information
  • 1VA Portland Health Care System and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA. Electronic address: mettenp@ohsu.edu.
  • 2VA Portland Health Care System and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA.
  • 3VA Portland Health Care System and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA.
  • 4VA Portland Health Care System and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA.
  • 5VA Portland Health Care System and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA.
  • 6VA Portland Health Care System and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA.
  • 7VA Portland Health Care System and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA.

Abstract

Despite acceptance that risk for alcohol-use disorder (AUD) has a large genetic component, the identification of genes underlying various components of risk for AUD has been hampered in humans, in part by the heterogeneity of expression of the phenotype. One aspect of AUD is physical dependence. Alcohol withdrawal is a serious consequence of alcohol dependence with multiple symptoms, many of which are seen in multiple species, and can be experienced over a wide-ranging time course. In the present three studies, we developed a battery of withdrawal tests in mice, examining behavioral symptoms from multiple domains that could be measured over time. To permit eventual use of the battery in different strains of mice, we used male and female mice of a genetically heterogeneous stock developed from intercrossing eight inbred strains. Withdrawal symptoms were assessed using commonly used tests after administration of ethanol in vapor for 72 continuous hours. We found significant effects of ethanol withdrawal versus air-breathing controls on nearly all symptoms, spanning 4 days following ethanol vapor inhalation. Withdrawal produced hypothermia, greater neurohyperexcitability (seizures and tremor), anxiety-like behaviors using an apparatus (such as reduced transitions between light and dark compartments), anhedonia (reduced sucrose preference), Straub tail, backward walking, and reductions in activity; however, there were no changes in thermal pain sensitivity, hyper-reactivity to handling, or anxiety-like emergence behaviors in other apparatus. Using these data, we constructed a refined battery of withdrawal tests. Individual differences in severity of withdrawal among different tests were weakly correlated at best. This battery should be useful for identifying genetic influences on particular withdrawal behaviors, which should reflect the influences of different constellations of genes.

Published by Elsevier Inc.

PMID

29427828

Others

Publication Type: Journal Article


This article is licensed under the the National Library of Medicine License. It uses material from the PubMed National Library of Medicine Data.


Last Modified:   2016-03-27


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