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Sunday, June 18th, 2017
Table of Contents

1 Introduction
5 PMID
 [F] Diseases Research  / PubMed Research Articles  /
Dual-target screening of bioactive components from traditional Chinese medicines by hollow fiber-based ligand fishing combined with liquid chromatography-mass spectrometry.

PubMed

 

Resource

Journal of pharmaceutical and biomedical analysis 2017 Jun 17; 143()

Authors

Chen L1; Wang X2; Liu Y3; Di X4;

Author Information
  • 1School of Pharmacy, Shenyang Pharmaceutical University,103 Wenhua Road, Shenyang 110016, PR China.
  • 2School of Pharmacy, Shenyang Pharmaceutical University,103 Wenhua Road, Shenyang 110016, PR China.
  • 3School of Pharmacy, Shenyang Pharmaceutical University,103 Wenhua Road, Shenyang 110016, PR China.
  • 4School of Pharmacy, Shenyang Pharmaceutical University,103 Wenhua Road, Shenyang 110016, PR China. Electronic address: dixin63@hotmail.com.

Abstract

A novel strategy was developed for dual-target screening of bioactive components from traditional Chinese medicines (TCMs). This strategy was based on the use of low-cost microporous hollow fibers filled with target enzymes as baits to "fish out" the ligands in TCM extracts, followed by identification of the ligands dissociated from the target-ligand complexes by liquid chromatography-mass spectrometry. Ganjiang Huangqin Huanglian Renshen Decoction (GHHRD), a classical TCM prescription for diabetes treatment, was chosen as a model sample to evaluate the feasibility of the proposed strategy. Three bioactive components were successfully screened out from GHHRD. Coptisine was identified as the ligand of α-glucosidase and baicalin as the ligand of angiotensin-converting enzyme (ACE). Berberine was found to be a dual inhibitor of α-glucosidase and ACE. The results were further verified by enzyme inhibitory assay and molecular docking simulation. The study suggested that our developed strategy would be a powerful tool for screening bioactive components from multi-component and multi-target TCMs.

Copyright © 2017 Elsevier B.V. All rights reserved.

PMID

28623809

Others

Publication Type: Journal Article


This article is licensed under the the National Library of Medicine License. It uses material from the PubMed National Library of Medicine Data.


Last Modified:   2016-03-27


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